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unpaired t test with equal sem  (GraphPad Software Inc)


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    GraphPad Software Inc unpaired t test with equal sem
    Stk33 KI/KI male mice were subfertile and the count and motility of sperm reduced. A , schematic diagram of CRISPR/Cas9 targeting strategy. The sgRNAs were designed within exon 13 of Stk33 , and stop codon was detected in Stk33 KI/KI mice by Sanger sequencing. B , photo of WT and Stk33 KI/KI testes. C , average testis weight/body weight in WT and Stk33 KI/KI mice. (N = 3). D , average number of pups per litter from WT and Stk33 KI/KI male mice. (N = 6). E , Stk33 mRNA expression levels in WT and Stk33 KI/KI testes. (N = 3). F , Western blot assay indicated that truncated STK33 protein was expressed in Stk33 KI/KI mice. G , sperm count in single cauda epididymis from WT and Stk33 KI/KI mice. (N = 3). H and I , average percentages of motile spermatozoa ( H ) and progressively motile spermatozoa ( I ) from WT and Stk33 KI/KI mice. (N = 3). All data are presented as mean ± <t>SEM</t> and analyzed <t>by</t> <t>unpaired</t> 2-tailed Student’s t test. NS, not significant; ∗ p < 0.05, ∗∗ p < 0.01. Stk33, serine/threonine kinase 33.
    Unpaired T Test With Equal Sem, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/unpaired+t+test+with+equal+sem/pmc10245119-54-10-11?v=GraphPad+Software+Inc
    Average 90 stars, based on 1 article reviews
    unpaired t test with equal sem - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "STK33 Phosphorylates Fibrous Sheath Protein AKAP3/4 to Regulate Sperm Flagella Assembly in Spermiogenesis"

    Article Title: STK33 Phosphorylates Fibrous Sheath Protein AKAP3/4 to Regulate Sperm Flagella Assembly in Spermiogenesis

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1016/j.mcpro.2023.100564

    Stk33 KI/KI male mice were subfertile and the count and motility of sperm reduced. A , schematic diagram of CRISPR/Cas9 targeting strategy. The sgRNAs were designed within exon 13 of Stk33 , and stop codon was detected in Stk33 KI/KI mice by Sanger sequencing. B , photo of WT and Stk33 KI/KI testes. C , average testis weight/body weight in WT and Stk33 KI/KI mice. (N = 3). D , average number of pups per litter from WT and Stk33 KI/KI male mice. (N = 6). E , Stk33 mRNA expression levels in WT and Stk33 KI/KI testes. (N = 3). F , Western blot assay indicated that truncated STK33 protein was expressed in Stk33 KI/KI mice. G , sperm count in single cauda epididymis from WT and Stk33 KI/KI mice. (N = 3). H and I , average percentages of motile spermatozoa ( H ) and progressively motile spermatozoa ( I ) from WT and Stk33 KI/KI mice. (N = 3). All data are presented as mean ± SEM and analyzed by unpaired 2-tailed Student’s t test. NS, not significant; ∗ p < 0.05, ∗∗ p < 0.01. Stk33, serine/threonine kinase 33.
    Figure Legend Snippet: Stk33 KI/KI male mice were subfertile and the count and motility of sperm reduced. A , schematic diagram of CRISPR/Cas9 targeting strategy. The sgRNAs were designed within exon 13 of Stk33 , and stop codon was detected in Stk33 KI/KI mice by Sanger sequencing. B , photo of WT and Stk33 KI/KI testes. C , average testis weight/body weight in WT and Stk33 KI/KI mice. (N = 3). D , average number of pups per litter from WT and Stk33 KI/KI male mice. (N = 6). E , Stk33 mRNA expression levels in WT and Stk33 KI/KI testes. (N = 3). F , Western blot assay indicated that truncated STK33 protein was expressed in Stk33 KI/KI mice. G , sperm count in single cauda epididymis from WT and Stk33 KI/KI mice. (N = 3). H and I , average percentages of motile spermatozoa ( H ) and progressively motile spermatozoa ( I ) from WT and Stk33 KI/KI mice. (N = 3). All data are presented as mean ± SEM and analyzed by unpaired 2-tailed Student’s t test. NS, not significant; ∗ p < 0.05, ∗∗ p < 0.01. Stk33, serine/threonine kinase 33.

    Techniques Used: CRISPR, Sequencing, Expressing, Western Blot

    Stk33 -/KI male mice were sterile and had a reduced number sperm with structural abnormalities. A , photo of WT and Stk33 -/KI testes. B , average testis weight/body weight in WT and Stk33 -/KI mice. (N = 5). C , average number of pups per litter from WT and Stk33 -/KI mice. (N = 4). D , sperm count in single cauda epididymis from WT and Stk33 -/KI mice. (N = 4). E - F , average percentages of motile ( E ) and progressively motile ( F ) spermatozoa from WT and Stk33 -/KI mice. (N = 6). G , Western blot assay indicated that Stk33 -/KI testes expressed truncated STK33 protein. H , H&E staining of spermatozoa from WT and Stk33 -/KI mice. The scale bar represents 10 μm. I , the percentage of abnormal sperm heads and tails in WT and Stk33 -/KI mice. (N = 4). J , PAS staining of testis sections from adult WT and Stk33 -/KI mice. The scale bar represents 10 μm. ( Red box in the upper right corner: magnification of abnormal spermatids; the red dotted line indicated decrease of spermatozooon flagella in stage VII-VIII seminiferous tubules of Stk33 -/KI mice). K , ultrastructure of cross sections of sperm head, middle piece, and principal piece from Stk33 -/KI mice. Abnormal heads and flagella are indicated by arrowheads . Pink arrowheads represent abnormal heads; green arrowheads represent abnormal mitochondria; yellow arrowheads represent abnormal outer dense fibers; blue arrowheads represent abnormal microtubules; and orange arrowheads represent abnormal fibrous sheaths (longitudinal columns and circumferential ribs). The scale bar represents 500 nm. All data are presented as mean ± SEM and analyzed by unpaired 2-tailed Student’s t test. NS, not significant; ∗ p < 0.05, ∗∗∗ p < 0.001. CR, circumferential ribs; D, diplotene; ES, elongated spermatids; FS, fibrous sheaths; L, leptotene; LC, longitudinal columns; N, nucleus; M, mitochondria; MT, microtubules; ODF, outer dense fibers; P, pachytene; PAS, periodic acid-Schiff; RS, round spermatids; Stk33, serine/threonine kinase 33; Z, Zygotene.
    Figure Legend Snippet: Stk33 -/KI male mice were sterile and had a reduced number sperm with structural abnormalities. A , photo of WT and Stk33 -/KI testes. B , average testis weight/body weight in WT and Stk33 -/KI mice. (N = 5). C , average number of pups per litter from WT and Stk33 -/KI mice. (N = 4). D , sperm count in single cauda epididymis from WT and Stk33 -/KI mice. (N = 4). E - F , average percentages of motile ( E ) and progressively motile ( F ) spermatozoa from WT and Stk33 -/KI mice. (N = 6). G , Western blot assay indicated that Stk33 -/KI testes expressed truncated STK33 protein. H , H&E staining of spermatozoa from WT and Stk33 -/KI mice. The scale bar represents 10 μm. I , the percentage of abnormal sperm heads and tails in WT and Stk33 -/KI mice. (N = 4). J , PAS staining of testis sections from adult WT and Stk33 -/KI mice. The scale bar represents 10 μm. ( Red box in the upper right corner: magnification of abnormal spermatids; the red dotted line indicated decrease of spermatozooon flagella in stage VII-VIII seminiferous tubules of Stk33 -/KI mice). K , ultrastructure of cross sections of sperm head, middle piece, and principal piece from Stk33 -/KI mice. Abnormal heads and flagella are indicated by arrowheads . Pink arrowheads represent abnormal heads; green arrowheads represent abnormal mitochondria; yellow arrowheads represent abnormal outer dense fibers; blue arrowheads represent abnormal microtubules; and orange arrowheads represent abnormal fibrous sheaths (longitudinal columns and circumferential ribs). The scale bar represents 500 nm. All data are presented as mean ± SEM and analyzed by unpaired 2-tailed Student’s t test. NS, not significant; ∗ p < 0.05, ∗∗∗ p < 0.001. CR, circumferential ribs; D, diplotene; ES, elongated spermatids; FS, fibrous sheaths; L, leptotene; LC, longitudinal columns; N, nucleus; M, mitochondria; MT, microtubules; ODF, outer dense fibers; P, pachytene; PAS, periodic acid-Schiff; RS, round spermatids; Stk33, serine/threonine kinase 33; Z, Zygotene.

    Techniques Used: Sterility, Western Blot, Staining



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    unpaired t test with equal sem  (GraphPad Software Inc)
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    GraphPad Software Inc unpaired t test with equal sem
    Stk33 KI/KI male mice were subfertile and the count and motility of sperm reduced. A , schematic diagram of CRISPR/Cas9 targeting strategy. The sgRNAs were designed within exon 13 of Stk33 , and stop codon was detected in Stk33 KI/KI mice by Sanger sequencing. B , photo of WT and Stk33 KI/KI testes. C , average testis weight/body weight in WT and Stk33 KI/KI mice. (N = 3). D , average number of pups per litter from WT and Stk33 KI/KI male mice. (N = 6). E , Stk33 mRNA expression levels in WT and Stk33 KI/KI testes. (N = 3). F , Western blot assay indicated that truncated STK33 protein was expressed in Stk33 KI/KI mice. G , sperm count in single cauda epididymis from WT and Stk33 KI/KI mice. (N = 3). H and I , average percentages of motile spermatozoa ( H ) and progressively motile spermatozoa ( I ) from WT and Stk33 KI/KI mice. (N = 3). All data are presented as mean ± <t>SEM</t> and analyzed <t>by</t> <t>unpaired</t> 2-tailed Student’s t test. NS, not significant; ∗ p < 0.05, ∗∗ p < 0.01. Stk33, serine/threonine kinase 33.
    Unpaired T Test With Equal Sem, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/unpaired+t+test+with+equal+sem/pmc10245119-54-10-11?v=GraphPad+Software+Inc
    Average 90 stars, based on 1 article reviews
    unpaired t test with equal sem - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    Stk33 KI/KI male mice were subfertile and the count and motility of sperm reduced. A , schematic diagram of CRISPR/Cas9 targeting strategy. The sgRNAs were designed within exon 13 of Stk33 , and stop codon was detected in Stk33 KI/KI mice by Sanger sequencing. B , photo of WT and Stk33 KI/KI testes. C , average testis weight/body weight in WT and Stk33 KI/KI mice. (N = 3). D , average number of pups per litter from WT and Stk33 KI/KI male mice. (N = 6). E , Stk33 mRNA expression levels in WT and Stk33 KI/KI testes. (N = 3). F , Western blot assay indicated that truncated STK33 protein was expressed in Stk33 KI/KI mice. G , sperm count in single cauda epididymis from WT and Stk33 KI/KI mice. (N = 3). H and I , average percentages of motile spermatozoa ( H ) and progressively motile spermatozoa ( I ) from WT and Stk33 KI/KI mice. (N = 3). All data are presented as mean ± SEM and analyzed by unpaired 2-tailed Student’s t test. NS, not significant; ∗ p < 0.05, ∗∗ p < 0.01. Stk33, serine/threonine kinase 33.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: STK33 Phosphorylates Fibrous Sheath Protein AKAP3/4 to Regulate Sperm Flagella Assembly in Spermiogenesis

    doi: 10.1016/j.mcpro.2023.100564

    Figure Lengend Snippet: Stk33 KI/KI male mice were subfertile and the count and motility of sperm reduced. A , schematic diagram of CRISPR/Cas9 targeting strategy. The sgRNAs were designed within exon 13 of Stk33 , and stop codon was detected in Stk33 KI/KI mice by Sanger sequencing. B , photo of WT and Stk33 KI/KI testes. C , average testis weight/body weight in WT and Stk33 KI/KI mice. (N = 3). D , average number of pups per litter from WT and Stk33 KI/KI male mice. (N = 6). E , Stk33 mRNA expression levels in WT and Stk33 KI/KI testes. (N = 3). F , Western blot assay indicated that truncated STK33 protein was expressed in Stk33 KI/KI mice. G , sperm count in single cauda epididymis from WT and Stk33 KI/KI mice. (N = 3). H and I , average percentages of motile spermatozoa ( H ) and progressively motile spermatozoa ( I ) from WT and Stk33 KI/KI mice. (N = 3). All data are presented as mean ± SEM and analyzed by unpaired 2-tailed Student’s t test. NS, not significant; ∗ p < 0.05, ∗∗ p < 0.01. Stk33, serine/threonine kinase 33.

    Article Snippet: Data were analyzed using an unpaired t test with equal SEM (GraphPad Prism 9, https://www.graphpad.com ).

    Techniques: CRISPR, Sequencing, Expressing, Western Blot

    Stk33 -/KI male mice were sterile and had a reduced number sperm with structural abnormalities. A , photo of WT and Stk33 -/KI testes. B , average testis weight/body weight in WT and Stk33 -/KI mice. (N = 5). C , average number of pups per litter from WT and Stk33 -/KI mice. (N = 4). D , sperm count in single cauda epididymis from WT and Stk33 -/KI mice. (N = 4). E - F , average percentages of motile ( E ) and progressively motile ( F ) spermatozoa from WT and Stk33 -/KI mice. (N = 6). G , Western blot assay indicated that Stk33 -/KI testes expressed truncated STK33 protein. H , H&E staining of spermatozoa from WT and Stk33 -/KI mice. The scale bar represents 10 μm. I , the percentage of abnormal sperm heads and tails in WT and Stk33 -/KI mice. (N = 4). J , PAS staining of testis sections from adult WT and Stk33 -/KI mice. The scale bar represents 10 μm. ( Red box in the upper right corner: magnification of abnormal spermatids; the red dotted line indicated decrease of spermatozooon flagella in stage VII-VIII seminiferous tubules of Stk33 -/KI mice). K , ultrastructure of cross sections of sperm head, middle piece, and principal piece from Stk33 -/KI mice. Abnormal heads and flagella are indicated by arrowheads . Pink arrowheads represent abnormal heads; green arrowheads represent abnormal mitochondria; yellow arrowheads represent abnormal outer dense fibers; blue arrowheads represent abnormal microtubules; and orange arrowheads represent abnormal fibrous sheaths (longitudinal columns and circumferential ribs). The scale bar represents 500 nm. All data are presented as mean ± SEM and analyzed by unpaired 2-tailed Student’s t test. NS, not significant; ∗ p < 0.05, ∗∗∗ p < 0.001. CR, circumferential ribs; D, diplotene; ES, elongated spermatids; FS, fibrous sheaths; L, leptotene; LC, longitudinal columns; N, nucleus; M, mitochondria; MT, microtubules; ODF, outer dense fibers; P, pachytene; PAS, periodic acid-Schiff; RS, round spermatids; Stk33, serine/threonine kinase 33; Z, Zygotene.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: STK33 Phosphorylates Fibrous Sheath Protein AKAP3/4 to Regulate Sperm Flagella Assembly in Spermiogenesis

    doi: 10.1016/j.mcpro.2023.100564

    Figure Lengend Snippet: Stk33 -/KI male mice were sterile and had a reduced number sperm with structural abnormalities. A , photo of WT and Stk33 -/KI testes. B , average testis weight/body weight in WT and Stk33 -/KI mice. (N = 5). C , average number of pups per litter from WT and Stk33 -/KI mice. (N = 4). D , sperm count in single cauda epididymis from WT and Stk33 -/KI mice. (N = 4). E - F , average percentages of motile ( E ) and progressively motile ( F ) spermatozoa from WT and Stk33 -/KI mice. (N = 6). G , Western blot assay indicated that Stk33 -/KI testes expressed truncated STK33 protein. H , H&E staining of spermatozoa from WT and Stk33 -/KI mice. The scale bar represents 10 μm. I , the percentage of abnormal sperm heads and tails in WT and Stk33 -/KI mice. (N = 4). J , PAS staining of testis sections from adult WT and Stk33 -/KI mice. The scale bar represents 10 μm. ( Red box in the upper right corner: magnification of abnormal spermatids; the red dotted line indicated decrease of spermatozooon flagella in stage VII-VIII seminiferous tubules of Stk33 -/KI mice). K , ultrastructure of cross sections of sperm head, middle piece, and principal piece from Stk33 -/KI mice. Abnormal heads and flagella are indicated by arrowheads . Pink arrowheads represent abnormal heads; green arrowheads represent abnormal mitochondria; yellow arrowheads represent abnormal outer dense fibers; blue arrowheads represent abnormal microtubules; and orange arrowheads represent abnormal fibrous sheaths (longitudinal columns and circumferential ribs). The scale bar represents 500 nm. All data are presented as mean ± SEM and analyzed by unpaired 2-tailed Student’s t test. NS, not significant; ∗ p < 0.05, ∗∗∗ p < 0.001. CR, circumferential ribs; D, diplotene; ES, elongated spermatids; FS, fibrous sheaths; L, leptotene; LC, longitudinal columns; N, nucleus; M, mitochondria; MT, microtubules; ODF, outer dense fibers; P, pachytene; PAS, periodic acid-Schiff; RS, round spermatids; Stk33, serine/threonine kinase 33; Z, Zygotene.

    Article Snippet: Data were analyzed using an unpaired t test with equal SEM (GraphPad Prism 9, https://www.graphpad.com ).

    Techniques: Sterility, Western Blot, Staining